Product Description
- Gel Dimensions: 10.0 cm (wide)x 15.0 cm (long)
- Maximum Sample Capacity: 80 samples (4 combs, 20 samples each)
- Buffer Capacity: 500 ml
- Distance Between Electrodes: 27.0 cm
- Maximum Voltage: 200 V
- Comes with: vented lid, UVT casting tray, casting fixture, two 2.0 mm x 16 tooth combs, bubble level, buffer ports, adjustable feet, and power cords
IBI’s MP-1015 Multi-Purpose Gel Box (10 x 15 cm) is a general purpose unit for both extended high-resolution linear runs as well as economical quick screens. It is equipped with recirculation ports for procedures requiring buffer recirculation. The MP-1015 IBI electrophoresis unit is constructed of durable cast acrylic, which will withstand high voltages and extreme temperatures without cracking or warping, unlike most molded units. The MP-1015 can be transformed into a quick screening unit with samples resolved in 30-60 minutes. Resolution of restriction digestions, plasmid purification, and ligation reactions are all possible with short runs. The MP-1015 is also compatible with a number of analytical and preparative combs for every horizontal electrophoresis application.
The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the MP-1015 is 140 V. In a 1% TBE gel this translates into a run time of approximately one hour. Lower voltages may be used and as a general rule a 70 V run will take twice as long as a 140 V run. Higher voltages may be used to decrease run time. However, if the unit is being operated at higher voltages than 140 V the heat generated during electrophoresis may decrease sample resolution. Such artifacts may be avoided by running the unit in a cold room, recirculating buffer, or adding 1 X electrophoresis buffer “ice cubes” to keep the unit properly cooled. The suggested run parameters for the electrophoresis of RNA in agarose gels containing formaldehyde is 60-80 V.
SPECIFICATION
Unit dimensions: 7.0 cm (H) x 16.5 cm (W) x 30.5 cm (L)
Gel Dimensions: 10 cm (W) x 15 cm (L)
Max Sample Capacity: 80 samples (4 combs x 20 samples ea.)
Buffer Capacity: 500 ml
Distance between electrodes: 27.0 cm
Max volt.: 200 V
