Product Description
Specifications:
Principle: Filter Plate (silica membrane)
Sample size: up to 1 x 10 animal cells or 50 mg tissues / preparation
Processing: centrifugation protocol or vacuum & centrifugation protocol
Operation time: < within 1 hr/ 96 preparation
RNA Binding capacity: up to 75 µg/ well
Elution volume: 50 ~ 75 µl
Important Notes:
1. Make sure everything is RNase-free when handling this kit.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when andling these buffers.
3. Caution: ß-mercaptoethanol is hazardous to human health. perform the procedures involving ß-M in a chemical fume hood.
4. Add RNase-free ethanol (96~100%) to Wash Buffer 1 and Wash Buffer 2 when first use.
5. Prepare RNase-free DNase 1 reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) and make the final concentration of DNase I to 0.5 U/ μl.
6. Equipments required:
• For centrifugation protocol: A centrifuge is required, capable of 5,600 ~ 6,000 X g, with a swing -bucket rotor and the adaptor for 96-well plates.
• For vacuum protocol: A vacuun manifold for 96-well plate and a vaccum source reached to 15 inches Hg are required. (Alternative): If using centrifugation for Elution Step (STEP 9), a centrifuge equiment is required, capable of 5,600 ~ 6,000 X g, with a swing -bucket rotor and the adaptor for 96-well plate.
Additional Requirements:
1. ß-mercaptoethanol (ß-Me)
2. 96 ~100 % RNase - free ethanol
3. 70 % RNase - free ethanol
FATRE 96001 | FATRE 96002 | FATRE 96004 | |
Lysis Buffer | 60 ml | 120 ml | 120 ml x 2 |
Wash Buffer 1 (concentrate) | 55 ml | 110 ml | 110 ml x 2 |
Wash Buffer 2 (concentrate) | 25 ml | 50 ml | 50 ml x 2 |
RNase-Free Water | 15 ml | 30 ml | 30 ml x 2 |
Filter Plate (96-Well RNA plate) | 1 plate | 2 plate | 4 plate |
Collection Plate (96-Well 2 ml Plate) | 4 plates | 8 plates | 16 plates |
Elution Plate (96-Well PCR plate) | 1 plate | 2 plates | 4 plates |
Adhesive Film | 2 pcs | 4 pcs | 8 pcs |
Stored:
Store at room temperature (15~ 25 ℃) for 2 year.
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