Product Description
- Electrophoresis buffer containing Tris, Acetate, and EDTA
- Makes 1L of 25X TAE Buffer Solution
TAE (Tris / Acetate / EDTA) buffer is commonly used in low voltage DNA electrophoresis applications. It’s relatively low buffering capacity will become exhausted in extended or current electrophoresis runs making buffer recirculation necessary in such runs. Potential advantages of TAE over TBE buffer include superior resolution of supercoiled DNA and slightly faster migration of double stranded DNA fragments.
The 25 X TAE Buffer Pouch can be mixed with 1 L of ddi water to make 1 L of 25 X TAE Buffer Solution. The pH level does not need to be adjusted.
Physical Specifications
CAS#: N/A
pH (1 X Solution @ 25ºC): 8.2-8.4
Refractive Index (20X Solution): 1.330-1.350
Recommended Use
TAE buffer systems are commonly used in low voltage DNA electrophoresis applications. It’s relatively low buffering capacity will become exhausted in extended or high current electrophoresis, making buffer recirculation necessary in such runs. Potential advantages of TAE over TBE buffers include superior resolution of supercoiled DNA, and slightly faster migration of ds linear DNA fragments.
