MLP - Multiplex Urinary Tract Infection Assay including ABX Resistance, 96 well, 3 panels per plate (Breakaway Plates)

Product Description

The Urinary Tract Plus Simplicity PanelTM is an in-vitro multiplex real-time polymerase chain reaction (RT-PCR) assay for the qualitative identification of nucleic acids from organisms frequently found in the urinary tract. This method is highly accurate, extremely sensitive, and is used to identify organisms by amplifying and detecting genetic material of pathogens in samples. The panel will aid in the research of causative agents of urinary tract infections and their prevalence.

The target organisms included in the panel are as follows:
Acinetobacter baumannii, Bacteroides fragilis, Candida albicans, Candida dubliniensis, Candida glabrata, Candida krusei, Candida parapsilosis, Candida tropicalis, Citrobacter freundii, Citrobacter koseri, Enterobacter cloacae, Enterococcus spp., Escherichia coli, Klebsiella aerogenes, Klebsiella oxytoca, Klebsiella pneumoniae, Morganella morganii, Mycoplasma genitalium, Mycoplasma hominis, Prevotella bivia, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Staphylococcus epidemidis, Staphylococcus saprophyticus, Streptococcus agalactiae (Group B), Streptococcus pyogenes (Group A), and Ureaplasma urealyticum.

Antibiotic Resistance Markers present
are as follows: β-lactamase (blaKPC), β-lactamase (CTX-M-Group 1), metallo-β-lactamase (blaNDM), Fluoroquinolones (qnr), Methicillin/Oxacillin (mecA), Sulfonamides (sul), Trimethoprim (dfrA), and Vancomycin (vanA, vanB).

Usage Information

	Reagent Storage and Use Guidelines
	Store all reagents at -25°C to -15°C Do not freeze-thaw plates more than 3 times.
	The Following is Included in the Kit
	96-well PCR plate pre-loaded with the Urinary Tract Plus Microbiota Simplicity PanelTM assays and positive  control. Negative control assay is plated but negative control is user supplied.
	The Following is Supplied by the User: Materials
	Extracted Sample(s) qPCR optical film Sealer for optical film Negative Control
	Equipment
	Manual defrost -20°C freezer Laminar Flow or PCR Dead Air Box for general plate setup. Do not use Laminar Flow for infectious samples Pipettes and appropriate filtered pipette tips Plate Vortex [recommend Vortex Genie 2 (Model G560) with the 3-inch platform and rubber cover] Plate centrifuge Lab utility knife
	Instrumentation
	CFX96 Touch Real-Time PCR Detection System (or equivalent)

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Limitations of Use
Molecular Designs products are for Research Use Only and intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Disclaimer - Use of PCR and Patent

This product is for basic PCR and is outside of any valid US patents assigned to Hoffman La-Roche.