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Electrophoresis and Blotting
Horizontal and vertical electrophoresis systems, power supplies, blotting equipment, gel documentation, agarose, acrylamide and protein markers.
Complete workflows for nucleic acid and protein separation.
Horizontal or vertical?
Horizontal (submarine) systems run agarose gels for DNA and RNA. Simple to pour, forgiving, and standard for PCR product checks, restriction digests and plasmid verification.
Vertical systems run polyacrylamide gels for proteins and high-resolution nucleic acid work. Higher resolution, but require cassette assembly and careful buffer handling.
Agarose percentage and resolution
Gel percentage determines the size range you can resolve:
- 0.7% — 800 bp to 10 kb, for large fragments and genomic DNA
- 1.0% — 400 bp to 8 kb, the general-purpose choice
- 1.5% — 200 bp to 3 kb, common for PCR products
- 2.0% and above — below 500 bp, for small amplicons and primer-dimer resolution
Power supply safety
Electrophoresis runs at voltages that are genuinely dangerous. Use supplies with no-load detection, ground leak detection and sudden-load-change shutoff. Never open a tank lid while the run is active, and confirm the supply has fully discharged before handling electrodes. Electrophoresis equipment has caused fatalities — these are not formalities.
Blotting and detection
Wet, semi-dry and dry transfer each trade time against efficiency. Wet transfer remains the most reliable for large proteins; semi-dry is faster for routine work. Nitrocellulose gives lower background, PVDF binds more protein and tolerates stripping and reprobing. Match your membrane to whether you plan to reprobe the blot.
Setting up a gel room or replacing a system? Call +1 305 600 0444.