Product Description
CAS# 151-21-3
- Ionic detergent used to denature proteins
SDS is an ionic detergent used to denature proteins in hybridization, nucleic acid purification, and electrophoresis buffer systems. SDS is also used to dissociate nucleic acid protein complexes in DNA extraction protocols, to disrupt cell membranes, and to prepare prehybridization and/or hybridization solutions.
To make a 10% SDS solution, dissolve 100 g of SDS in 900 ml of ddi water. Heat to 68⁰ C to assist dissolution. Adjust the pH to 7.2 by adding a few drops of HCl. Adjust the total volume to 1 L with ddi water, then dispense into aliquots.
Physical Specifications
CAS#: 151-21-3
Molecular Formula: C12H25NaO4S
Molecular Weight: 288.38
Purity: Min. 99.0%
A260 (3%, water): Max. 0.1
A280 (3%, water): Max. 0.1
Chloride: Max. 0.1%
Phosphate: Max. 0.0005%
Heavy Metals (as Pb): Max. 0.0005%
Copper: Max. 0.0005%
Molecular Biology Specifications
DNase assay: None Dectected
RNase assay: None Dectected
Protease assay: None Dectected
