Product Description
Wnt-3a is a key member of the Wnt family of secreted glycoproteins that regulate essential processes in embryonic development, tissue regeneration, and stem cell maintenance. Wnt-3a activates the canonical Wnt/β-catenin signaling pathway by binding to Frizzled and LRP5/6 receptors, leading to β-catenin stabilization and transcriptional activation of Wnt target genes involved in cell proliferation, differentiation, and fate determination.
Wnt-3a is produced as a lipid-modified precursor and undergoes post-translational modifications critical for its secretion and bioactivity. It plays a vital role in anterior-posterior patterning, neural crest development, and limb formation. In adult tissues, Wnt-3a continues to influence cell renewal and homeostasis, particularly in the intestinal epithelium and hematopoietic systems. Recombinant Wnt-3a is widely used in vitro to maintain pluripotency in stem cells, drive mesodermal and endodermal lineage differentiation, and study cancer, developmental biology, and tissue engineering applications.
Applications
- Pluripotent Stem Cell Maintenance: Supports self-renewal and maintenance of human embryonic and induced pluripotent stem cells via activation of canonical Wnt/?-catenin signaling
- Directed Differentiation: Promotes mesoderm and endoderm lineage specification during early-stage differentiation protocols in stem cell cultures
- Organoid Development: Essential for the growth and maturation of organoids, including intestinal, hepatic, and neural models, by mimicking in vivo Wnt signaling environments
- Cancer and Developmental Biology Studies: Used to investigate Wnt pathway dysregulation in tumor progression and to model signaling pathways involved in embryogenesis and tissue patterning.Tissue repair and fibrosis in regenerative medicine. BMP signaling modulation in developmental studies
Product Specifications
| Storage | -20°C |
| Storage Information | Avoid freeze/thaw cycles |
| Species | Human |
| Amino Acid Sequence | APLGYFLLL CSLKQALGSY PIWWSLAVGP QYSSLGSQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGI KIGIQECQHQ FRGRRWNCTT VHDSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GTAAICGCSS RHQGSPGKGW KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH LKCKCHGLSG SCEVKTCWWS QPDFRAIGDF LKDKYDSASE MVVEKHRESR GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS SHGIDGCDLL CCGRGHNARA ERRREKCRCV FHWFCCYVSCQ ECTRVYDVHT CK |
| Molecular Weight | 37.4 kDa |
| Class | Recombinant |
| Purity | > 95% by SDS-PAGE gel |
| Endotoxin Concentration | < 1 EU/µg |
| Biologically Active | Yes |
| Biological Activity | The biological activity is confirmed by its ability to induce alkaline phosphatase production be MC3T3-E1 mouse preosteoblast cells. |
| Form | Lyophilized |
| Shipping Conditions | Ambient |
| Reconstitution | Reconstitute with PBS + 0.1% BSA + 0.1% CHAPS |
