- Electrophoresis buffer solution containing Tris and Glycine
Tris-Glycine Buffer is used for polyacrylamide electrophoresis. It can be used in both native and denaturing (SDS) polyacrylamide gel electrophoresis of proteins. The versatility of Tris-Glycine Buffer enables the accurate separation of proteins in the range of 5 – 500 kDa.
With a Tris-Glycine buffer, the protein range for a 10% and 16% polyacrylamide gel is 20-250 kDa.
1 X 0.192 M Glycine
pH (1 X Solution @ 25°C): 8.3 to 8.7
Tris-Glycine Buffer is used for polyacrylamide electrophoresis (Laemmil 1970). Can be use in both native and denaturing (SDS) polyacrylamide gel electrophoresis of proteins The versatility of Tris-Glycine buffer enables the accurate separation of protein in the range of 5 to 500 KDa