Product Description
Solis BioDyne 10x Lysis Buffer
Universal Lysis Buffer for Rapid DNA Extraction
The Solis BioDyne 10x Lysis Buffer is a highly efficient, universal lysis reagent designed for the rapid extraction of DNA from a wide variety of sample types. It is specifically engineered to handle the most inhibitor-rich matrices commonly encountered in applied sciences, such as plant seeds and leaves. The product has been rigorously validated on challenging matrices including soy, salad leaves, spinach, wheat, mung beans, and maize. The resulting DNA lysate is fully compatible with allele-specific genotyping experiments, such as those performed with the HOT SolisAcura™ Cassette-based Genotyping Mix.
Designed to streamline laboratory workflows and maximize efficiency, this buffer enables complete DNA extraction in under 5 minutes utilizing a convenient one-tube format. This approach significantly minimizes the risk of sample loss and cross-contamination. The extracted DNA is immediately suitable for demanding downstream analyses—including direct qPCR, genotyping, GMO, and pathogen detection—without the need for additional cleanup steps. Furthermore, the lysate demonstrates exceptional compatibility; adding up to 50% of the lysate to a reaction does not inhibit the amplification process, and the extracted lysate can be safely stored at -20°C for over a month.
- Ultra-Rapid Extraction: Complete DNA lysis from sample to PCR-ready template takes no more than 5 minutes.
- Contamination Prevention: The one-tube lysis format eliminates transfer steps, actively preventing both contamination and sample loss.
- Inhibitor Resistance: Delivers superior results with highly challenging, inhibitor-rich agricultural samples like wheat, soy, and spinach.
- Direct Downstream Compatibility: Extracted DNA is fully ready for direct detection of viral and bacterial DNA, genotyping, and direct qPCR without additional purification.
- High Reaction Tolerance: Assays can accommodate up to 50% of the lysate volume in the final reaction mixture without suffering from inhibition.
- Exceptional Stability: Stable at room temperature for up to 1 month and withstands 10 freeze-thaw cycles, enabling convenient ambient temperature shipping and flexible reaction setup.
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | Universal Lysis Buffer (10x concentration) |
| Extraction Time | Under 5 minutes |
| Validated Matrices | Soy, salad leaves, spinach, wheat, mung beans, maize |
| Downstream Applications | Direct qPCR, genotyping, GMO & pathogen detection, viral/bacterial DNA detection |
| Routine Storage | -20°C (-28°C to -18°C) |
| Temporary Storage & Shipping | Room temperature (+25°C) for up to 1 month |
| Refrigerated Stability | +4°C (+2°C to +8°C) for up to 12 months |
| Freeze-Thaw Stability | 10 cycles |
Product Variants
| Catalog Number (SKU) | Pack Size (Volume) | Number of Preps |
|---|---|---|
| 05-95-00001 | 1.0 ml | 100 extractions |
| 05-95-00004 | 4.0 ml | 400 extractions |
| 05-95-00020 | 20.0 ml | 2000 extractions |