Product Description
The kit provides a very simple, fast and cost effective technique to isolate high quality RNA. Using one simple protocol, high yield of purified RNA can be isolated from various sources including blood, culture cells, leukocytes, tissue and plant. MagaBio sample processing is based on proprietary magnetizable particles--MagaBio. The pure RNA can be applied extensively in RT-PCR, Northern hybridization, mutant analysis, RNAi and the others.
According to the special interaction, use MagaBio nucleic acid separation system with a general protocol---sample processing, MagaBio adsorption, washing and elution, and can go high-throughput.
Principle and Advantage
The nucleic acid in the sample is released under the action of Lysis Buffer and PK, and the released nucleic acid specifically binds to the magnetic beads.
The magnetic bead particles that bind the nucleic acid are captured by the magnetic material, and the contaminants are removed through multiple washing processes. Finally, the RNA is eluted and collected from the magnetic beads under the action of the eluent.
Apparatus and materials to be prepared by the user
- Bioer NPA-32P purification instrument
- Water bath or Dry bath
- Vortex mixer
- Deep well plate centrifuge
- Absolute alcohol (For BSC53S1B and BSC53M1B)
Important Notes
- Add the alcohol (as the volume marked on bottle label) to the DNase Stop Buffer and Wash Buffer, and then mix them well.
- MagaBio Reagent should be mixed well (particles are uniformly suspended) every time.
- MagaBio plus total RNA Purification Kit has been optimized for use with fresh whole blood and leukocytes, frozen blood samples cannot be used.
- Proper microbiological, asceptic technique should always be followed when working with RNA. All plasticware and glassware should be free of RNase contamination. Keep the tubes closed during the procedure, work quickly, and keep the purified RNA on ice until used.
- If the room temperature is too low ,we can put the ML Buffer at 45℃ warm bath for preheating.
Kit Contents
| Cat# | |
| Components | 32 Tests |
| ML Buffer | 12.8 mL |
| Binding Buffer | Pre- packed 32 Individual Strips |
| DNase Stop Buffer | |
| Wash Buffer I | |
| Elution Buffer | |
| MagaBio Reagent | |
| Handbook | 1 |
1. The kit should be stored at 2°C -25°C for the duration of the shelf life.
2. Do not use beyond the expiration date printed on the box.
3. The kit can be transported at room temperature.
4. The kit can be stored for up to 12 months if all components are kept on the conditions above.
