Amiprox ProtiPrep Pop2 Mini Spin Column for HSA/IgG Plasma Depletion, 25 Columns/Case

Product Description

ProtiPrep™ Pop2 Mini Spin Column — Deplete. Enrich. Discover.

Plasma is a goldmine of biological insight, but also one of the most challenging fluids to analyze. Highly abundant proteins such as human serum albumin (HSA) and immunoglobulin G (IgG) make up nearly 70 % of total plasma protein content, masking the low-abundance biomarkers that matter most.

In just 10 minutes, ProtiPrep™ Pop2 removes over 97 % of HSA and IgG from only 10 µL of human plasma, leaving the sample ready for downstream proteomic analysis. A patented centrifugal-flow microspin device is combined with prefilled buffer compartments in the lid, so the complete depletion workflow takes place in a single tube — no buffer preparation, no column conditioning, no transfer steps.

Key Features

• Depletes the two most abundant plasma proteins, HSA and IgG, in a single 10-minute workflow
• Requires only 10 µL of human plasma per preparation
• Prefilled buffer compartment integrated into the lid — no separate reagent handling
• Patented centrifugal flow design for reproducible, low-variability recovery
• Increases the depth of the detectable plasma proteome for LC-MS/MS discovery work
• Simple bench workflow using a standard microcentrifuge

Performance Data

• Depletion efficiency (ELISA): 97.27 % HSA and 98.62 % IgG. Pooled plasma from 134 donors, n = 61 samples processed in three technical replicates, measured with the HSA ELISA Kit (KE00076, Proteintech) and the Human IgG Total ELISA Kit (Thermo Fisher).
• Proteome depth (LC-MS/MS): approximately 100 additional distinct protein groups identified in depleted samples (n = 9) versus undepleted neat plasma (n = 3) — 327 + 103 versus 328 proteins. Acquired on an Orbitrap Exploris 480 (Thermo Fisher Scientific), analyzed with R 4.5.2 and SpectroPipeR 0.4.6.1, counting only proteins identified with at least two unique peptides. Data generated by ProTec Diagnostic GmbH, Greifswald, Germany.
• SDS-PAGE: strong reduction of the HSA (66.5 kDa) and IgG heavy-chain (50 kDa) and light-chain (25 kDa) bands in depleted samples, with specific capture confirmed in the bead fraction (Novex™ WedgeWell™ 4–20 % Tris-Glycine gel).

Workflow

1. Add sample (10 µL plasma)
2. Screw the lid on to release the prefilled buffer
3. Tap 10×
4. Shake for 10 seconds
5. Incubate 5 minutes
6. Centrifuge
7. Take out the column
8. Collect the sample — ready for downstream analysis

Ordering Information

Manufactured by Amiprox GmbH, Stiegstück 34, 22339 Hamburg, Germany. Distributed in the United States by Pro Lab Supply Corp.

Note: For Research Use Only. Not for use in diagnostic procedures.